Molecular diagnostics tests play a crucial role in modern medicine, utilizing advanced techniques such as PCR, NGS, and gene expression analysis to analyze DNA and RNA for genetic disorders, infectious diseases, oncology, and pharmacogenetics.
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DNA microarrays, a revolutionary technology for studying gene expression and genomic analysis. Explore the principles behind DNA microarrays, their applications in research and diagnostics, and their role in understanding genetic diseases and personalized medicine
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Discover the power of reverse transcription polymerase chain reaction (RT-PCR), a versatile molecular biology technique used to amplify and detect RNA molecules. Explore the principles behind RT-PCR, including reverse transcription of RNA into complementary DNA (cDNA) and subsequent PCR amplification.
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Discover the power of Polymerase Chain Reaction (PCR), a revolutionary technique for amplifying specific DNA sequences. Explore the principles behind PCR, including denaturation, annealing, and extension, and understand how it enables the exponential replication of DNA.
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SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) is a commonly used technique in biochemistry and molecular biology to separate and analyze proteins based on their molecular weight.
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Agarose gel electrophoresis is a widely employed technique in molecular biology and genetics for the separation and analysis of DNA molecules based on their size.
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Introduction: Electrophoresis is a common molecular laboratory technique by which a mixture of charged molecules is separated according to size under the influence of an electric field. It is used to identify, quantify, and purify nucleic acid fragments. Samples are placed into wells of an agarose or acrylamide gel and exposed to an electric field,
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High-performance liquid chromatography (HPLC) is a powerful analytical technique used in chemistry, biochemistry, and pharmaceutical sciences for the separation, identification, and quantification of chemical compounds in complex mixtures.Fluorescence detectors
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Western blotting, also known as immunoblotting, is a widely used laboratory technique in molecular biology and biochemistry for the detection and analysis of specific proteins within a complex mixture of proteins. It allows researchers to identify and quantify target proteins based on their size and antigenic properties.
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Southern blotting is a laboratory technique used in molecular biology to detect and analyze specific DNA sequences within a complex mixture of DNA molecules. It enables researchers to identify and characterize DNA fragments of interest based on their complementary base pairing.
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