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  • Prion Diseases: Mechanism, Pathogenesis, and Human Disorders (CJD, Kuru, FFI)

    Introduction to Prions: When a misfolded prion enters a healthy organism, it causes already-existing, correctly folded proteins to transform into the form of a misfolded prion. Therefore, other normal proteins misfold into prion form using prions as a template. Prion disorders, also known as transmissible spongiform encephalopathies, are a class of neurodegenerative illnesses that are

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  • Fasciola hepatica: Introduction, clinical manifestation and diagnosis

    Introduction to Fasciola hepatica: Fasciola hepatica is a hermaphroditic trematode with a complex life-cycle that includes an intermediate (snail) and definitive (mammalian) host. The earliest known trematode or fluke was F. hepatica.  However, it was initially found in sheep rather in humans. The disease, known as liver rot, is brought on by consuming metacercaria from

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  • Dengue Virus: Introduction, Morphology, Genome, Replication Cycle, and Pathogenesis

    Introduction to Dengue Virus: Dengue is a mosquito-borne disease caused by the skin inoculation any of four antigenically different serotypes of the Dengue virus (DENV 1-4), characterized by wide spectrum of illness ranging from asymptomatic to severe forms. As the name ‘breakbone fever’, the patient complains of pain in the back, joint, muscles and the

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  • Clinical Specimen Selection, Collection, Transport, and Storage for Microbiological Diagnosis

    General Considerations in Specimen Collection The vitals for specimen collection: Requisition The following details should be included in the laboratory request, or “lab slip”: Specimen selection and Collection: Timing of Specimen Collection: Transport of Clinical Specimens: Storage Guidelines for Different Specimen Types: Criteria for Specimen Rejection: SPECIMEN SELECTION POSSIBLE BACTERIA COLLECTION EQUIPMENT TRANSPORT Wounds, Abscesses

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  • Shigella: Introduction, characteristics, virulence factors, and pathogenesis

    Introduction to Shigella: Shigella are the cause of bacillary dystentery exclusively facultative intracellular pathogens in intestine of humans and other primates. Shigella are slender (1-3 × 0.5 µm in size) Gram-negative rods belonging to the family Enterobacteriaceae. They are non-motile bacterium, non–sporulating and non-capsule formers. The genus Shigella comprises four different species; S. dysenteriae, S.flexneri,

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  • Selection, Collection, and Preservation of Stool Samples for Accurate Diagnosis of Intestinal Parasitic Infections

    Selection of Stool Samples: Collection of Stool Samples: Principles of Proper Sample Collection Recommended Materials and Precautions Preservation of Stool Samples: Principle and Importance of Preservation Types of Preservatives and Their Uses: Schaudinn’s Fixative Advantages Disadvantages PVA (Polyvinyl Alcohol) Advantages Disadvantages MIF (Merthiolate-Iodine-Formalin) Advantages Disadvantages Diluted Formalin Advantages Disadvantage

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