Sanger sequencing is a method of DNA sequencing that was developed by Frederick Sanger in the 1970s. It is a chain termination method that uses modified nucleotides, called dideoxynucleotides, to stop the synthesis of a growing DNA strand.
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The primary goal of extracting nucleic acids is for downstream applications such as PCR, sequencing, cloning, genetic analysis or forensic applications.
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In electroporation, an electric current is applied to a living surface, such as the skin or a cell membrane that open pores or channels through which is used to introduce foreign DNA or other molecules into cells.
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Introduction: A pipette is a laboratory tool that is used to measure and transfer small volumes of liquids. It is a tube with a calibrated tip and a plunger that allows the user to draw a specific volume of liquid into the pipette and then dispense it. Pipettes are commonly used in scientific research and
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Introduction: Fragile X syndrome (FXS) is a genetic disorder that causes a variety of developmental issues such as learning impairments and cognitive impairment. It is caused by a mutation in the FMR1 gene, which is responsible for producing a protein called fragile X mental retardation protein (FMRP). This protein plays a critical role in the
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Learn about Nanopore sequencing, a real-time DNA sequencing method using nanopores and electrical currents for accurate and rapid analysis
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DNA barcoding is a powerful technique revolutionizing biodiversity research and species identification. It involves analyzing short gene sequences, such as the mitochondrial cytochrome c oxidase subunit I (COI), to distinguish and classify organisms. By comparing these DNA barcodes to reference databases, researchers can accurately identify species, even those with morphological similarities or at different life stages.
The process begins with specimen collection, followed by DNA extrac
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Introduction: Cystic fibrosis (CF) is a hereditary condition that impacts the respiratory, digestive, and reproductive systems. It is caused by a mutation in the gene that codes for a protein called cystic fibrosis transmembrane conductance regulator (CFTR). This protein regulates the movement of salt and water in and out of cells, and is involved in
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Introduction: Principle: Types: Methods: There are several different methods of NGS, each with its own strengths and limitations. These are just a few examples of the many methods of NGS that are available. The choice of method depends on the specific research question and the type of data that is needed. Steps: Sequencing data can
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Gene sequencing is the process of determining the precise order of nucleotides within a DNA molecule or fragment, providing a wealth of information about the genetic makeup of an organism or sample.
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