Introduction: Isoelectric point (pI) is the pH at which the net electric charge on a molecule/protein is zero. At its pI, the molecule/protein is electrically neutral that the molecule at its pI exists as a zwitterion with an equal number of positive charges (from protonated amino groups) and negative charges (from deprotonated carboxyl groups). At […]
Aim: To estimate the concentration of protein using Biuret method. Principle: The is a colorimetric estimation technique that is specific for proteins and peptides used for estimation of protein. The coppers salt reacts with the two more peptide bonds under alkaline condition result in purple complex. The intensity of the formed color complex is proportional
Aim: To estimate the concentration of protein using Bradford Method. Principle: The Bradford protein assay is a commonly used method for estimating the concentration of proteins in a sample. Generally, it is based on the binding of Coomassie Brilliant Blue (CBB) dye to proteins, resulting in a shift its maximum absorbance maximum from 465 nm
Aim: To separate green leaf pigments (Spinach leaves) by Thin Layer chromatography (TLC) method. Introduction: It is a type of planar chromatography, in which the stationary phase is a thin layer of adsorbent particles attached to a solid plate. A small amount of sample/analyte is applied (spotted) near the bottom of the plate (an imaginary
Introduction: The DNS (3,5-dinitrosalicylic acid) method is commonly used for the quantitative estimation of reducing sugars, including glucose. This method is based on the reaction of reducing sugars with DNS reagent, which results in the formation of a coloured compound that can be measured spectrophotometrically. Principle: The principle behind the DNS (3,5-dinitrosalicylic acid) method for
Introduction: Benefits of immobilized enzyme: Fig: Application of immobilised enzyme Properties of carrier matrix for ideal immobilization: Biocompatibility: The carrier matrix needs to be biocompatible in order to maintain the activity, stability, and conformation of the specific enzyme. It shouldn’t cause the enzyme to become denaturized or inactive. Chemical Stability: The matrix should be chemically
Ketone bodies, including acetoacetate, beta-hydroxybutyrate, and acetone, are metabolic byproducts that play a crucial role in energy production during periods of carbohydrate restriction or prolonged fasting.
Phytohormones, also known as plant hormones or plant growth regulators, are chemical messengers that regulate the growth, development, and response to environmental stimuli in plants.
Photosynthesis is the process by which green plants, algae, and some bacteria convert light energy from the sun into chemical energy in the form of organic compounds, primarily carbohydrates.
Optogenetics is a technique that uses light to control the activity of cells or neurons that have been genetically modified to respond to light.