Introduction: cDNA library is a population of bacterial transformation or phage lysates in which each mRNA isolated from an organism or tissues is represented as its cDNA insertion in a plasmid or a phage vector. The frequency of a specific cDNA in such a library would ordinarily depend on the frequency of the concerned mRNA […]
Construction of a cDNA Library: Introduction, Steps, Applications Read More »
Introduction: A restriction enzyme is a nuclease enzyme that breaks down DNA sequences at random or specified recognition regions known as restriction sites. In bacteria, restriction enzymes interact with modification enzymes to form a combination system (restriction + modification system) that methylates the bacterial DNA. Methylation of bacterial DNA at the recognition sequence often protects
Restriction endonucleases (REase): Introduction, Features, Types, Applications Read More »
Introduction: The process of finding new drugs involves combining clinical, translational, experimental, and computational models. Drug design is the inventive approach that aims to design drugs whose target molecules may be receptor (45%), an enzyme (20% of cases), ion channel (5% of cases) DNA (2% of cases), nuclear receptor (2% of cases) or unknown (7% of cases).
Drug Designing: Steps, Procedure, Process, and Delivery Read More »
Introduction: In mammals, aging is a complex process that is accompanied by a progressive degeneration of tissues and organs which results in the loss of the ability to retain normal homeostasis. Aging is caused by accumulated damage to DNA, RNA, proteins, and lipids which in turn inhibits the ability of the cell to function normally
DNA Damage theories: Introduction, Theories, Mechanisms Read More »
Introduction: It is an invitro technique used to mutate one or more bases within a plasmid. Likewise, this procedure is to create specific, targeted changes in double stranded plasmid DNA with modified sequences for examining the importance of specific residues in protein structure and function. It is also called site-specific mutagenesis or oligonucleotide-directed mutagenesis. There
Site-Directed Mutagenesis (SDM): Introduction, Methods, Applications Read More »
Single-cell RNA sequencing (scRNA-seq) is a cutting-edge technology that allows researchers to profile gene expression in individual cells, providing unprecedented insights into cellular heterogeneity, differentiation, and disease.
Transcription factors are proteins that regulate gene expression by controlling the rate at which DNA is transcribed into RNA and play a crucial role in cellular processes.
A molecular biology approach used to introduce precise mutations into a DNA sequence is site-directed mutagenesis. Researchers can use this technique to investigate the effects of mutations on protein function and to create proteins with desired features.
Site-Directed Mutagenesis (SDM)- Introduction, Principle, Steps, Protocol, Applications Read More »
The Maxam-Gilbert method is a sequencing technique that was developed in the 1970s for determining the nucleotide sequence of DNA.
Rheumatoid arthritis (RA) is a chronic autoimmune condition that arises when our immune system incorrectly targets own body’s tissues. It is a significant public health concern that can cause significant disability and reduced quality of life.
Rheumatoid Arthritis- Symptoms, Types, Pathogenesis, Diagnosis, Medications, Prevention Read More »
