Sanger sequencing is a method of DNA sequencing that was developed by Frederick Sanger in the 1970s. It is a chain termination method that uses modified nucleotides, called dideoxynucleotides, to stop the synthesis of a growing DNA strand.
Sanger Sequencing- Principle, Protocol, Applications, Limitations Read More »
The Philadelphia chromosome is a result of a reciprocal translocation between the long arms of chromosomes 9 and 22 t (9;22) (q34; q11). It was given the name of the town where it was found.
The Philadelphia Chromosome- Leukemia, Translocation, Diagnostic Tests Read More »
The primary goal of extracting nucleic acids is for downstream applications such as PCR, sequencing, cloning, genetic analysis or forensic applications.
Nucleic Acid (DNA/RNA)- Detection, Purity, Quantitation, Applications Read More »
In electroporation, an electric current is applied to a living surface, such as the skin or a cell membrane that open pores or channels through which is used to introduce foreign DNA or other molecules into cells.
Electroporation-Definition, Types, Process, Applications Read More »
Introduction: History: The concept of digital PCR (dPCR) was first described in a 1996 paper by Scherer and colleagues, who proposed using microfluidic devices to partition a sample into thousands of small compartments and perform PCR on each compartment. The first commercial dPCR instrument, the Bio-Rad iCycler iQ, was introduced in 2003. Since then, dPCR
Digital PCR: Definition, Principle, Instrumentation, Steps, Applications Read More »
lncRNA-miRNA Gene Expression Regulation in Cancer
RNA Splicing- Definition, Process, Mechanism, Types, Errors, Importance Read More »
Post-transcriptional modifications refer to a variety of molecular alterations that occur to RNA molecules after transcription, playing crucial roles in gene expression regulation.
Post-transcriptional modifications (PTMs)- Steps, Regulations, Processing Read More »
Introduction: Fragile X syndrome (FXS) is a genetic disorder that causes a variety of developmental issues such as learning impairments and cognitive impairment. It is caused by a mutation in the FMR1 gene, which is responsible for producing a protein called fragile X mental retardation protein (FMRP). This protein plays a critical role in the
Fragile X syndrome (FXS)- Introduction, Frequency, Causes, Diagnosis, Treatment Read More »
Learn about Nanopore sequencing, a real-time DNA sequencing method using nanopores and electrical currents for accurate and rapid analysis
Nanopore Sequencing- Principle, Steps, Device, Applications Read More »
DNA barcoding is a powerful technique revolutionizing biodiversity research and species identification. It involves analyzing short gene sequences, such as the mitochondrial cytochrome c oxidase subunit I (COI), to distinguish and classify organisms. By comparing these DNA barcodes to reference databases, researchers can accurately identify species, even those with morphological similarities or at different life stages.
The process begins with specimen collection, followed by DNA extrac
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