Cell Interaction and Integration: Cell surface, Cell junctions, Cell signals

Introduction: Cell are to survive in varying conditions; therefore, they should be able to get stimuli and respond to differences caused in the environment. Such responses are based on the integration of several components or systems mediated by its membranes, receptors, chemicals, effectors, pH differences, etc. They perform appropriate activities that enhance the chances of […]

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Inverted Microscope: Introduction, Principle, Parts, Applications, Maintenance

Introduction: It is similar to compound microscopes except that their components are placed in inverted order. The specimen is viewed from below and the source of light and condenser are positioned above the specimen. Light is directed down onto the stage/specimen in this design, and specimens in larger laboratory containers, such as flasks and Petri

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X-Ray Crystallography: Introduction, Workflow, Bragg’s Law, Applications

Introduction: X-ray crystallography is a tool used for determining the atomic and molecular structure of a crystal. The underlying principle is that the crystalline atoms cause a beam of X-rays to diffract into many specific directions. Principle: The atoms of crystal diffract X-rays to several specific directions, and the intensity and angle of the diffracted

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Enzyme Immobilization/ Immobilized enzyme: Introduction, Methods, Factors, Kinetics,Applications

Introduction: Benefits of immobilized enzyme: Fig: Application of immobilised enzyme Properties of carrier matrix for ideal immobilization: Biocompatibility: The carrier matrix needs to be biocompatible in order to maintain the activity, stability, and conformation of the specific enzyme. It shouldn’t cause the enzyme to become denaturized or inactive. Chemical Stability: The matrix should be chemically

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In Vitro Fertilization (IVF)

In Vitro Fertilization (IVF): List of IVF & Fertility Center in Nepal

In vitro fertilization (IVF) is a medical procedure used to help people or couples who are having trouble getting pregnant. In vitro fertilization (IVF) includes fertilizing an egg with sperm outside the body in a laboratory dish, followed by the implantation of the fertilized embryo into the uterus. The primary objective of IVF is to

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Site-Directed Mutagenesis (SDM): Introduction, Methods, Applications

Introduction: It is an invitro technique used to mutate one or more bases within a plasmid. Likewise, this procedure is to create specific, targeted changes in double stranded plasmid DNA with modified sequences for examining the importance of specific residues in protein structure and function. It is also called site-specific mutagenesis or oligonucleotide-directed mutagenesis. There

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Lytic cycle of bacteriophage (Virus): Introdcution, and Stages

Introduction: Bacteriophages are viruses that only infect bacteria which is also known as phages. They have a unique structural and life cycle and are highly abundant and diverse in nature such as water, soil, and the human body. It can infect and replicate within bacterial cells. The term “bacteriophage” originates from the Greek words “bacterio,”

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Viruses- Introduction, History, Features, Classification, Diseases

Introduction: A virus is a microscopic infectious agent that can cause diseases which is able to utilize the synthetic machinery of the living cell of another organism for its multiplication. Virus are the smallest living organism ranging from about 20nm to 300nm that are about 50 times smaller than bacteria approximately.  They are too small

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Protocol for Gram staining: Background, Purpose, Equipment, Steps

Background: Gram staining is a key technique in microbiology that allows bacteria to be classified into two groups: Gram-positive and Gram-negative. This staining method, developed by Hans Christian Gram in the late 1800s, gives vital insights into bacterial cell wall composition and has become a cornerstone of bacterial identification and classification. The differential staining is

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Bacteria: History, Taxonomy, Structure, Morphology, Functions, Nutrition

Introduction: Bacteria are single-celled microorganisms that belong to the domain Bacteria. They are one of the most abundant and diverse kinds of life on Earth, and can be found in a variety of habitats. These microscopic organisms have a distinct cellular structure and a remarkable ability for adaptation and survive in a variety of environments,

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