Enzyme Immobilization/ Immobilized enzyme: Introduction, Methods, Factors, Kinetics,Applications

Introduction: Benefits of immobilized enzyme: Fig: Application of immobilised enzyme Properties of carrier matrix for ideal immobilization: Biocompatibility: The carrier matrix needs to be biocompatible in order to maintain the activity, stability, and conformation of the specific enzyme. It shouldn’t cause the enzyme to become denaturized or inactive. Chemical Stability: The matrix should be chemically […]

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In Vitro Fertilization (IVF)

In Vitro Fertilization (IVF): List of IVF & Fertility Center in Nepal

In vitro fertilization (IVF) is a medical procedure used to help people or couples who are having trouble getting pregnant. In vitro fertilization (IVF) includes fertilizing an egg with sperm outside the body in a laboratory dish, followed by the implantation of the fertilized embryo into the uterus. The primary objective of IVF is to

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Site-Directed Mutagenesis (SDM): Introduction, Methods, Applications

Introduction: It is an invitro technique used to mutate one or more bases within a plasmid. Likewise, this procedure is to create specific, targeted changes in double stranded plasmid DNA with modified sequences for examining the importance of specific residues in protein structure and function. It is also called site-specific mutagenesis or oligonucleotide-directed mutagenesis. There

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Lytic cycle of bacteriophage (Virus): Introdcution, and Stages

Introduction: Bacteriophages are viruses that only infect bacteria which is also known as phages. They have a unique structural and life cycle and are highly abundant and diverse in nature such as water, soil, and the human body. It can infect and replicate within bacterial cells. The term “bacteriophage” originates from the Greek words “bacterio,”

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Viruses- Introduction, History, Features, Classification, Diseases

Introduction: A virus is a microscopic infectious agent that can cause diseases which is able to utilize the synthetic machinery of the living cell of another organism for its multiplication. Virus are the smallest living organism ranging from about 20nm to 300nm that are about 50 times smaller than bacteria approximately.  They are too small

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Protocol for Gram staining: Background, Purpose, Equipment, Steps

Background: Gram staining is a key technique in microbiology that allows bacteria to be classified into two groups: Gram-positive and Gram-negative. This staining method, developed by Hans Christian Gram in the late 1800s, gives vital insights into bacterial cell wall composition and has become a cornerstone of bacterial identification and classification. The differential staining is

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Bacteria: History, Taxonomy, Structure, Morphology, Functions, Nutrition

Introduction: Bacteria are single-celled microorganisms that belong to the domain Bacteria. They are one of the most abundant and diverse kinds of life on Earth, and can be found in a variety of habitats. These microscopic organisms have a distinct cellular structure and a remarkable ability for adaptation and survive in a variety of environments,

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Evaporation of Water from the Leaf Surface

Transpiration- Types, Measurement, Mechanism, Factors, Significance, Advantages

Introduction: The process of the loss of water from the internal tissues of the living plants in the form of vapor the exposed parts of the plants is regarded as transpiration. Plant absorbs large amount of water from the soil through the root hair but only small amount is utilized in different metabolic activities. Types:

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Osmosis- Introduction, Types, Solution, Osmotic pressure, Factors, Significance

Introduction: Osmosis is biological process that refers to the movement of free water molecules from a region of high concentration to a region of low concentration across a partially-permeable membrane until they are evenly spread out. It may define as the diffusion of the solvent molecules into the solution through a semipermeable membrane. Types of

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