Plasmids- Definition, Properties, Structure, Types, Functions, Examples
Plasmids are used for a variety of reasons in recombinant DNA technology.
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MOLECULAR BIOLOGY
Polymerase Chain Reaction (PCR)- Principle, Steps, Applications
Discover the power of Polymerase Chain Reaction (PCR), a revolutionary technique for amplifying specific DNA sequences. Explore the principles behind PCR, including denaturation, annealing, and extension, and understand how it enables the exponential replication of DNA.
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CRISPR-Cas9- Introduction, Mechanism, Diagnosis, Applications
Introduction: What Is CRISPR-Cas9? CRISPR-Cas9 as a Adaptive Immune: Invading bacteriophages are protected against bacteria by the CRISPR-Cas9 system. As spacers within the bacterial genome’s CRISPR locus, Cas1 and Cas2 incorporate a brief section of the viral genome after infection. The CRISPR locus is expressed as pre-CRNA and tracrRNA upon re-infection with the same virus.
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SDS-PAGE- Principle, Components, Steps, Applications
SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) is a commonly used technique in biochemistry and molecular biology to separate and analyze proteins based on their molecular weight.
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Agarose Gel Electrophoresis- Principle, Types, Components, Steps, Applications
Agarose gel electrophoresis is a widely employed technique in molecular biology and genetics for the separation and analysis of DNA molecules based on their size.
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Electrophoresis- Principle, Types, Workflow, Applications
Introduction: Electrophoresis is a common molecular laboratory technique by which a mixture of charged molecules is separated according to size under the influence of an electric field. It is used to identify, quantify, and purify nucleic acid fragments. Samples are placed into wells of an agarose or acrylamide gel and exposed to an electric field,
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Western Blotting- Principle, Steps, Extraction, Applications
Western blotting, also known as immunoblotting, is a widely used laboratory technique in molecular biology and biochemistry for the detection and analysis of specific proteins within a complex mixture of proteins. It allows researchers to identify and quantify target proteins based on their size and antigenic properties.
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Southern Blotting- Principle, Steps, Detection, Applications
Southern blotting is a laboratory technique used in molecular biology to detect and analyze specific DNA sequences within a complex mixture of DNA molecules. It enables researchers to identify and characterize DNA fragments of interest based on their complementary base pairing.
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Translation Or Protein Synthesis- Components, Stages, Inhibitors
Translation is the cellular process in which the genetic information encoded in mRNA is used to synthesize proteins.
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DNA Fragments detection in the Blood: New Diagnostic Test for TB
Myobacteria were the first bacterial infections to be discovered in humans, and they continue to cause serious illnesses till date. Robert Koch described the bacterium that causes tuberculosis in 1882. Since then, a vast number of mycobacterial species that cause pulmonary and extrapulmonary infections in humans and wildlife have been reported. Two primary pathogenic organisms,
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