Transcription vs. Replication: Differences, RNA Polymerase Structure, and Transcription Termination Mechanisms

Introduction to Transcription: Transcription is the biological process in which an RNA strand is generated based on a DNA template, with the reaction being facilitated by the enzyme RNA polymerase. Similarities Between Transcription and Replication: Differences between transcription and replication:  Characteristics Replication Transcription Definition DNA replication is the biological process of duplication of the double […]

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Tight Junction Disruption in the Human Gut: Mechanisms by Helicobacter pylori and Other Enteric Pathogens

Introduction to Tight Junctions: The epithelium and endothelium segregate the tissue compartments in a host body, and the host is protected from the outside world. Tight junctions selectively control what gets into and out of the cell.  Tight Junctions are the intercellular contacts that prevent harmful entities present in the lumen of the stomach from

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Molecular Events During Bacterial Growth: A Comprehensive Phase-Wise Overview of Physiological and Genetic Changes

Introduction to Bacterial Growth: Bacterial growth is a complex process that involves biosynthetic reactions including synthesis of cell constituents and metabolites and anabolic reactions that involves the breakdown of cell constituents and metabolites.  Bacteria growing in a closed system typically go through four distinct stages: the lag phase, exponential (log) phase, stationary phase, and decline

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Cryptosporidiosis: Comprehensive Overview of Pathogenesis, Clinical Manifestations, and Diagnostic Approaches

Introduction to Cryptosporidiosis: Cryptosporidia are coccidian parasites that have tropism for the microvillous region of the digestive epithelium and epithelium of respiratory organs of vertebrates. In immunocompetent individuals, Cryptosporidium parvum resolves on its own after causing a short-term diarrhea. However, in the immunocompromised patients, cryptosporidiosis can be life threatening. The host is infected by the

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Bacterial Growth and Exponential Kinetics: Understanding Growth Rate, Generation Time, and Continuous Culture Dynamics

Introduction to Bacterial Growth: Cells divide when they have gathered everything they require for growth. The population doubles during the exponential phase of expansion, which is characterized by rapid increase under ideal circumstances. During the exponential phase, every cell undergoes rapid, regular division through binary fission, leading to geometric (exponential) population expansion. The cells divide

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Cytokine Storm Syndrome (CSS) in COVID-19: Mechanisms, Clinical Impact, and Therapeutic Insights

Introduction- COVID-19 and Global Health Impact: One of the most pressing global health issues that humanity has encountered since World War II is the COVID-19 pandemic. With the exception of Antarctica, the virus has spread to every continent since it first appeared in Asia in 2020. Medical professionals and researchers were investigating the underlying mechanism

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Cyclospora cayetanensis: Lifecycle, Pathogenesis, Laboratory Detection, and Public Health Strategies

Introduction: Cyclospora is an intracellular, single-celled sporozoan with a diameter of around 8.6 μm. When someone consumes food or water contaminated with the parasite, they become infected. It cannot transmit from one person to another as the oocysts in the faecal matter require a certain days and temperature for sporulation and becoming infective. The infected

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Total Quality Management in microbiology laboratory

Introduction to Total Quality Management (TQM) in a microbiology laboratory: There are all the activities that effort to establish and maintain continued improvement in lab to do what it is intended to do i.e. clinical relevance achievement by delivery of high quality services to customer aiding in patient management. Lab organization: Organization is one of

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Potential Risks of Using Continuous Cell Lines (CCLs) in Viral Vaccine Development

Introduction to Cell Substrates in Vaccine Production: Cell substrates have been a never-ending source of concern about the potential for transmitting infectious diseases and/or cancer. The initial key decision in selecting cell substrates for vaccine production was to minimize the risk of cancer transmission through live viral vaccines. This led to the restriction of acceptable

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Immunodeficiency: Introduction, brief account of life cycle of HIV and immunodeficiency during HIV infection

Introduction to Immunodeficiency: Life Cycle of HIV: HIV infection is usually transmitted vertically from an infected mother to the baby via the placenta or by breastfeeding. Horizontally, HIV can be transmitted through activities like non-screened blood transplantation, use of infected needle sticks, needle stick injury, and unsafe sexual contact.  So, the virus can be transmitted

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