Mass spectrometry (MS): Introduction, Principle, Instrumentation, Applications

Introduction: It is an analytical tool which is used to measure the mass-to-charge ratio (m/z) of one or more molecules present in an analyte sample. Eventually, the measurement can be applied to calculate the molecular weight of the sample to quantify known compounds, and to determine structure and chemical properties of molecules. The preference for the term “mass […]

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Sterilization Techniques: Introduction, Physical and Chemical method, Applications

Introduction: Microorganisms are ubiquitous. Microbes grow and multiply under favorable conditions. In laboratory, for the purposes of study, they are allowed to grow and multiply. So, it always becomes necessary to remove or destroy microbes from materials or from areas because they are responsible for causing contamination, decay, and infection. This is the main object

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Electron Spin Resonance Spectroscopy (ESR): Introduction, Principle, Instrumentation, Applications

Introduction: It is a technique which us the spring property of an electron which are in resonance.  It is based on the paramagnetic behavior of an electron which are in resonance. All molecules have an atom and every atom have a nucleus. Every nucleus contains proton and neutron. Neutron is neutral and proton is positively

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Atomic Absorption Spectroscopy (AAS): Introduction, Principle, Instrumentation, Applications

Introduction: It is an analytical technique used to determine the concentration of certain elements, metal atoms/ions in given sample. Metals which consist of 75% of earth chemical elements sometime also make a content as a contaminant in several components (mine. Foods, water, etc) Therefore, this technique uses the principle in which atoms (and ions) can

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Estimation of Protein by Biuret Method: Principle, Procedure, Observation Table, Result

Aim: To estimate the concentration of protein using Biuret method. Principle: The is a colorimetric estimation technique that is specific for proteins and peptides used for estimation of protein. The coppers salt reacts with the two more peptide bonds under alkaline condition result in purple complex. The intensity of the formed color complex is proportional

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Estimation of Protein by Bradford Method:Principle, Procedure, Observation Table, Result

Aim: To estimate the concentration of protein using Bradford Method. Principle: The Bradford protein assay is a commonly used method for estimating the concentration of proteins in a sample. Generally, it is based on the binding of Coomassie Brilliant Blue (CBB)  dye to proteins, resulting in a shift its  maximum absorbance maximum from 465 nm

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Extrachromosomal Inheritance: Introduction, Plastid inheritance, Mitochondrial Inheritance, Maternal effect

Introduction: The genes of nuclear chromosome have different roles in inheritance, cellular metabolism, development and mutation of the organism in which they occur. The gens of nuclear chromosomes have a significant and key role in the inheritance of almost all characters from one generation to other generation. However, they alone cannot be considered as the

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Separation of Plant Pigments by Thin layer chromatography (TLC)

Aim: To separate green leaf pigments (Spinach leaves) by Thin Layer chromatography (TLC) method. Introduction: It is a type of planar chromatography, in which the stationary phase is a thin layer of adsorbent particles attached to a solid plate. A small amount of sample/analyte is applied (spotted) near the bottom of the plate (an imaginary

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Spectrophotometer: Introduction, Principle, Instrumentation, Types, Applications

Introduction: It is an instrument used to measure absorbance at various wavelengths which is composed of two units: a spectrometer and a photometer. Moreover, this is used to measures the number of photons (the intensity of light) absorbed after it passes through sample solution. Basically, the spectrometer is to use to produce light of any

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Extremophilic Microorganisms: Introduction, Types and Applications

Introduction: The microorganisms are present in every nitch on the earth, except volcano and vacuum. These microorganisms have high adaptability. The majority of the microorganisms survive and grow in normal conditions like room temperature, around neutral pH, etc. But some microorganism survives and grow in extreme conditions like very high or very low temperature, salt

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