MICROBIOLOGY

Total Quality Management in microbiology laboratory

Introduction to Total Quality Management (TQM) in a microbiology laboratory: There are all the activities that effort to establish and maintain continued improvement in lab to do what it is intended to do i.e. clinical relevance achievement by delivery of high quality services to customer aiding in patient management. Lab organization: Organization is one of […]

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Potential Risks of Using Continuous Cell Lines (CCLs) in Viral Vaccine Development

Introduction to Cell Substrates in Vaccine Production: Cell substrates have been a never-ending source of concern about the potential for transmitting infectious diseases and/or cancer. The initial key decision in selecting cell substrates for vaccine production was to minimize the risk of cancer transmission through live viral vaccines. This led to the restriction of acceptable

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Immunodeficiency: Introduction, brief account of life cycle of HIV and immunodeficiency during HIV infection

Introduction to Immunodeficiency: Life Cycle of HIV: HIV infection is usually transmitted vertically from an infected mother to the baby via the placenta or by breastfeeding. Horizontally, HIV can be transmitted through activities like non-screened blood transplantation, use of infected needle sticks, needle stick injury, and unsafe sexual contact.  So, the virus can be transmitted

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Ascariasis: Introduction, pathogenesis, diagnosis, prevention and control

Introduction: Ascaris lumbricoides belongs to class Phasmida of Phylum Aschelminthes or Nemathelminthes. It is commonly called a roundworm, attributed to its worm-like morphology. Because A. lumbricoides eggs can withstand a variety of environmental circumstances, a large number of eggs are generated per female worm, and inadequate sanitary conditions are common, particularly in developing and undeveloped

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Protein Secretion in Bacteria: Introduction, Sec and Tat Pathways, Signal Recognition Particle (SRP) Pathway

Introduction to Protein Secretion in Bacteria: Like all other organisms, bacteria have all the necessary cell structures that ensure their survival and proper functioning. The protective cell envelope can be two or three membranes, depending on the nature of the bacteria. Bacteria are protected from the environment by one or two membranes that prevent the

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Conventional and rapid methods in the laboratory diagnosis of pyrexia of unknown origin

Introduction to Pyrexia of Unknown Origin (PUO): Pyrexia of unknown origin (PUO) is a temperature above 38.3 degrees Celsius, lasting more than 3 weeks, and there is failure to reach a diagnosis despite a week of inpatient investigation.  PUO could be classic PUO (temperature >38.3 degrees Celsius for more than 3 weeks and evaluation of

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Biosecurity and Biosafety: Framework, Levels, Protocols, and Regulations

Introduction to Biosecurity and Biosafety: Biosecurity is the prevention of misuse, loss, theft and unauthorized access, and intentional release of harmful agents like pathogens, their toxins, and the equipment involved in doing so. The Biological and Toxin Weapons Convention was formed in 1975 to address the bad use of bioweapons. The biosecurity functions are at

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Bacterial invasion into the host: Zipper and Trigger mechanism

Introduction and overview of bacterial invasion: Living inside host cells helps bacterial pathogens to exploit the intracellular niche and nutrient-rich environment. Many pathogenic bacteria employ phagocytosis to enter the host cell. After entering the cells, these bacteria block phagolysosome formation, preventing lysis of the self. This conceals the bacteria from host defense. To infiltrate the

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Type II Hypersensitivity Reactions: Introduction, Mechanisms, Clinical Manifestations Blood Transfusion Reactions, Hemolytic Disease of the Newborn

Introduction to Hypersensitivity Reactions: When an individual has been immunologically primed, further contact with the antigen leads to a secondary immune response, boosting. However, if the antigen is present in relatively large amounts or if the humoral and cellular immune state is at a heightened level, the reaction may be excessive and may lead to

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Comparative Morphology and Life Cycle of Taenia solium and Taenia saginata: Key Differences and Similarities

Introduction: Tapeworms (Taenia solium and Taenia saginata) belong to the class Cestoda of phylum Platyhelminthes. Tapeworms are flat, parasitic animals with complex life cycles that infect animals, including humans. They are exclusively endoparasites. Comparison and contrast of Taenia solium and Taenia saginata in terms of morphology: Differences between Taenia solium and Taenia saginata: Differences in

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