Fluorescent probe-based PCR

RT-PCR- Definition, Principle, Components, Types, Steps, Applications

Discover the power of reverse transcription polymerase chain reaction (RT-PCR), a versatile molecular biology technique used to amplify and detect RNA molecules. Explore the principles behind RT-PCR, including reverse transcription of RNA into complementary DNA (cDNA) and subsequent PCR amplification.

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CRISPR-Cas9- Introduction, Mechanism, Diagnosis, Applications

Introduction: What Is CRISPR-Cas9? CRISPR-Cas9 as a Adaptive Immune: Invading bacteriophages are protected against bacteria by the CRISPR-Cas9 system. As spacers within the bacterial genome’s CRISPR locus, Cas1 and Cas2 incorporate a brief section of the viral genome after infection. The CRISPR locus is expressed as pre-CRNA and tracrRNA upon re-infection with the same virus.

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