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Digital PCR: Definition, Principle, Instrumentation, Steps, Applications

Introduction: History: The concept of digital PCR (dPCR) was first described in a 1996 paper by Scherer and colleagues, who proposed using microfluidic devices to partition a sample into thousands of small compartments and perform PCR on each compartment. The first commercial dPCR instrument, the Bio-Rad iCycler iQ, was introduced in 2003. Since then, dPCR

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Animal Cell- Definition, Structure, Types, Functions

Introduction: An animal cell is a type of eukaryotic cell that is characteristic of animals. Eukaryotic cells are characterized by the presence of a true nucleus, which contains the cell’s genetic material in the form of DNA. Animal cells also have several other membrane-bound organelles, such as mitochondria, which are responsible for generating energy for

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Fluorescent probe-based PCR

RT-PCR- Definition, Principle, Components, Types, Steps, Applications

Discover the power of reverse transcription polymerase chain reaction (RT-PCR), a versatile molecular biology technique used to amplify and detect RNA molecules. Explore the principles behind RT-PCR, including reverse transcription of RNA into complementary DNA (cDNA) and subsequent PCR amplification.

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