BIOCHEMISTRY

Introduction to Clinical Microbiology Laboratory Organization: A clinical microbiology lab performs analytical tests on human body substances for disease diagnosis, prevention and aids patient management for promotion of good health. Organization means an orderly structure making arrangements for achievement of purpose. In microbiology lab, the purpose is clinical relevance i.e., promotion of patient’s health.  Key […]

Objective: To determine the acid value of given fat sample Theory: Acid value is defined as the number of milligrams of potassium or sodium hydroxide that is required to neutralize the free fatty acids in 1 gram of fats or oil. It is a measure of the number of carboxylic acid group in a chemical

Introduction:  A biosensor is an analytical device, which employs a biological material to specifically interact an analyte; this interaction procedures some detectable physical change that is measured and converted into an electrical signal by a transducer. Finally, the electrical signal is amplified, interpreted, and displayed as analyte concentration in the solution/preparation. An analyte is a

In 1961, chemiosmotic theory was proposed by Peter D. Mitchell, a British biochemist, that suggested an electrochemical link between phosphorylation and cellular respiration. Later, in 1978, he was awarded a Nobel prize for chemistry for his work on the mechanism of synthesis of ATP across the inner mitochondrial membrane in cells. The process of chemical

Introduction: Types:  O- Glycosylation Occurs post- translationally at certain serine and threonine residue takes place in Golgi apparatus. O-linked oligosaccharides have a glycosidic bond to the oxygen atom of the hydroxyl group of amino acid including serine (Ser) or threonine (Thr) residues and N-Acetyl galactosamine (GalNAc) as the sugar at the reducing end of the

Introduction: It is also known as glyoxylate shunt (GS), was identified by Kornberg and Krebs in 1957, explaining how organisms could grow on acetate as the sole carbon source (Kornberg and Krebs, 1957). The glyoxylate cycle, an anabolic pathway, is a variation of the tricarboxylic acid cycle, is an anabolic pathway occurring in plants, bacteria,

Introduction: Isoelectric point (pI) is the pH at which the net electric charge on a molecule/protein is zero. At its pI, the molecule/protein is electrically neutral that the molecule at its pI exists as a zwitterion with an equal number of positive charges (from protonated amino groups) and negative charges (from deprotonated carboxyl groups). At

Principle: Activity of enzyme is defined as the micromoles of product formed per ml of enzyme solution under the optimum conditions. The determination of specific activity of enzyme involves two steps estimation of protein by F.C. Methos and estimation of product (glucose) by DNS method. The FC method is one of the best methods for

Aim: To estimate the concentration of protein using Biuret method. Principle: The is a colorimetric estimation technique that is specific for proteins and peptides used for estimation of protein. The coppers salt reacts with the two more peptide bonds under alkaline condition result in purple complex. The intensity of the formed color complex is proportional

Aim: To estimate the concentration of protein using Bradford Method. Principle: The Bradford protein assay is a commonly used method for estimating the concentration of proteins in a sample. Generally, it is based on the binding of Coomassie Brilliant Blue (CBB)  dye to proteins, resulting in a shift its  maximum absorbance maximum from 465 nm